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Study of Intercellular Adhesion Protein Expression in Staphylococcus aureus under Treatment of Phenolic Conjugated Copper Nanoparticles
Authors
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Sajjad Mohsin Irayyif
Department of Medical Basic Sciences, College of Dentistry, University of Wasit, Wasit, Iraq
Abstract
Staphylococcus aureus is one of the most prevalent infectious organisms that contribute to morbidity and mortality worldwide. This bacterial pathogen can cause a wide range of ailments, from pneumonia and sepsis to severe skin infections. There is presently no effective immunization against S. aureus infections, and antibiotic resistance makes treatment more challenging. The green biosynthesis of nanoparticles (NPs) utilizing biomaterials is a recent area of interest in nanotechnology and nanoscience. The goal of this work was to create phenolic conjugated copper nanoparticles (CuNPs), a unique use of nanotechnology. The investigation was conducted to determine the antibacterial activity against Staphylococcus aureus after characterizing using FTIR and UV-Vis absorption spectra. We discovered a distinctive signal at 365 nm that corresponds to the phenolic extract's surface plasmon resonance (SPR). Our findings supported the green produced CuNPs' possible antibacterial function.Additionally, we observed a noteworthy 84% suppression of biofilms in comparison to the positive control, rifampicin. We discovered that the icaA, icaB, caC, and icaD gene members were down-regulated as a percentage from the qPCR analysis. In conclusion, the environmentally friendly synthesis of CuNPs from pheolics can be a good option for clinical applications such drug delivery systems, pharmaceutical formulation, and biological applications.
Keywords:
Biofilm Copper nanoparticles MIC qPCR Spathodea campanulata Staphylococcus aureus
Article information
Journal
Journal of Life Science and Public Health
Volume (Issue)
1(2), (2025)
Pages
49-57
Published
Copyright
Copyright (c) 2025 Sajjad Mohsin Irayyif (Author)
Open access
This work is licensed under a Creative Commons Attribution 4.0 International License.
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References
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