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Review Article

In Vitro Sample Hemolysis in Chemistry Laboratories and Its Challenges

Authors

  • Taha S. Mostafa Mohamed Department of Medical Biochemistry, Assiut University, Assiut, Egypt

    drtsmohamed@gmail.com

Abstract

Hemolysis is an old and new challenging preanalytical cause of errors in chemistry laboratories, and there is a lot of controversy as regards it; also, it is the number one cause of sample rejection in chemistry laboratories. In this review, we try to spotlight the causes, how to detect hemolytic samples, how hemolysis can affect analytical assays, the biochemical effect of hemolysis on chemistry analytes, and how to reduce errors related to hemolysis. In this narrative review We aimed to understand the causes of invitro hemolysis, to clarify how to detect it precisely, to outline how hemolysis affect the chemistry lab results and its effect on clinical decision, when to accept or reject the hemolyzed chemistry sample and spotlight on how to reduce hemolysis and its effect on the chemistry results. We conclude that, Hemolysis interferes with numerous biochemical parameters, primarily potassium, AST, LDH and CK-MB, either by falsification or modification of the respective concentrations. The interference can be reduced by using standardized collection techniques, using HIL indices, and regular training of personnel. 

Keywords:

Hemolysis Hil Indices In-Vitro Hemolysis Preanalytical Errors

Article information

Journal

Journal of Medical Science, Biology, and Chemistry

Volume (Issue)

2(2), (2025)

Pages

186-191

Published

07-10-2025

How to Cite

Mohamed, T. S. M. (2025). In Vitro Sample Hemolysis in Chemistry Laboratories and Its Challenges. Journal of Medical Science, Biology, and Chemistry, 2(2), 186-191. https://doi.org/10.69739/jmsbc.v2i2.566

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