Therapeutic Administration of Thymoquinone on Potential Regulation of Folliculogenesis and Mice Estrus Cycle Activation

Abstract

The study of the evaluation of therapeutic potential administration of thymoquinone on mice estrus cycle function when exposed to the stressed environment condition has been carried out in the animal house at the College of Veterinary Medicine University of Wasit. Thirty mice received three different treatments: The control group received normal saline drenching while group members in the stress and thymoquinone groups received normal saline and ten mg/kg b.w. thymoqunone, respectively. Both groups stayed in (30±1°C) conditions for two weeks. Results indicated that thymoqunone significantly affected reproductive functions at physiological levels than the control group did. The ovaries were collected from all groups post-thymoquinone treatment through synthesized mice which received a mixture of xylazine 10 mg/kg b.w and kitamine 100 mg/kg b.w. The samples were maintained at liquid nitrogen for PCR analysis of stress-induced changes in ovaries tissue through inhibin hormone and HSP70 protein differentiation. The thymoquinone treatment at (10 mg/kg b.w.) created a highly meaningful change compared to the heated (30±1°C) stressed animals for the two-week trial.